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You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?

Answer :

After the addition of the first dye, the gram-positive bacteria will appear as purple.

Explanation:

The gram-positive bacteria has thecell wall made up of peptidoglycan. In Gram-positive bacteria the purple of crystal violet gram stains is retained by this thick peptidoglycan layer.

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