Answer :
The study that would most likely be used to investigate the hypothesis that South America and Africa was once part of the same continent is option A.
Comparing fossil records from both continents. Fossils that are found in different continents can be used to tell if the continents were once a supercontinent.
Continental drift is what causes the continents to separate. Finding fossils of different animals and plants in these two continents can tell that the continents were once a single continent.
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